Product Name
Nephrin (NPHS1), Polyclonal Antibody
Full Product Name
Nephrin (Phospho-Tyr1176) Antibody
Product Synonym Names
CNF; Nephrin; Nephrosis 1 congenital Finnish type; Nephrosis 1, congenital, Finnish type (nephrin); NPHN; NPHN_HUMAN; NPHS 1; Nphs1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor;
Product Gene Name
anti-NPHS1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60500
Species Reactivity
Human, Mouse
Specificity
Phospho-Nephrin(Phospho-Tyr1176) Antibody? detects endogenous levels of Nephrin only when phosphorylated at Tyr1176
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human Nephrin around the phosphorylation site of Tyr1176
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-NPHS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-NPHS1 antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-NPHS1 antibody
Western Blot (WB)
Application Notes for anti-NPHS1 antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-NPHS1 antibody
Western blot analysis of extracts of mouse kidney? tissue sample, using Phospho-Nephrin(Tyr1176) Antibody?.((13292)
NCBI/Uniprot data below describe general gene information for NPHS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004637.1
[Other Products]
NCBI GenBank Nucleotide #
NM_004646.3
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UniProt Primary Accession #
O60500
[Other Products]
UniProt Secondary Accession #
A6NDH2; C3RX61[Other Products]
UniProt Related Accession #
O60500[Other Products]
Molecular Weight
85[Similar Products]
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
NPHS1, nephrin
NCBI Official Symbol
NPHS1??[Similar Products]
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
??[Similar Products]
NCBI Protein Information
nephrin
UniProt Protein Name
Nephrin
UniProt Synonym Protein Names
Renal glomerulus-specific cell adhesion receptor
UniProt Gene Name
NPHS1??[Similar Products]
UniProt Synonym Gene Names
NPHN??[Similar Products]
NCBI Summary for NPHS1
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
UniProt Comments for NPHS1
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion ().
Research Articles on NPHS1
1. WHSC1L1-L acts as a histone methyltransferase in podocytes and regulates nephrin gene expression, which may in turn contribute to the integrity of the slit diaphragm of the glomerular filtration barrier.
Precautions
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Disclaimer
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