PRKAG3; protein kinase, AMP-activated, gamma 3 non-catalytic subunit; AMPKG3; 5'-AMP-activated protein kinase, gamma-3 subunit; AMP-activated protein kinase, non-catalytic gamma-3 subunit; AMPK gamma-3 chain; PRKAG3 antibody; protein kinase; AMP-activated; gamma 3 non-catalytic subunit antibody; AMPKG3 antibody; 5\1-AMP-activated protein kinase; gamma-3 subunit antibody; AMP-activated protein kinase; non-catalytic gamma-3 subunit antibody; AMPK gamma-3 chain antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-PRKAG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA)

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Preliminary experiments in Human Heart, Skeletal Muscle and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).

51,484 Da

protein kinase AMP-activated non-catalytic subunit gamma 3

5'-AMP-activated protein kinase subunit gamma-3

5'-AMP-activated protein kinase subunit gamma-3

AMPKG3; AMPK gamma3; AMPK subunit gamma-3??[Similar Products]

AAKG3_HUMAN

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]

AMPKG3: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Skeletal muscle, with weak expression in heart and pancreas. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: AMP-activated protein kinase complex; cytosol; extracellular space; nucleoplasm

Molecular Function: AMP-activated protein kinase activity; ATP binding; protein kinase binding

Biological Process: cell cycle arrest; fatty acid biosynthetic process; glucose transport; glycogen biosynthetic process; macroautophagy; protein amino acid phosphorylation

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