Npr2/ANPRB/ANP-B/ANPR-B/NPR-B/GC-B/Atrial natriuretic peptide receptor type B/Guanylate cyclase B

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of Npr2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761628 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Atrial natriuretic peptide receptor 2 (Npr2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Npr2. The ELISA analytical biochemical technique of the MBS761628 kit is based on Npr2 antibody-Npr2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Npr2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Npr2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Npr2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Npr2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Npr2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Npr2 can be calculated.

111,208 Da

natriuretic peptide receptor 2

atrial natriuretic peptide receptor 2

Atrial natriuretic peptide receptor 2

ANPRB; ANP-B; ANPR-B; NPR-B; GC-B??[Similar Products]

ANPRB_HUMAN

This gene encodes natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq, Jul 2008]

ANPB: natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. 21 mutations linked to acromesomelic dysplasia (skeletal malformation). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, dual-specificity (receptor); Membrane protein, integral; Kinase, protein; Guanylyl cyclase; EC 4.6.1.2; Nucleotide Metabolism - purine; Receptor, misc.; Protein kinase, RGC; Lyase; RGC group; RGC family

Chromosomal Location of Human Ortholog: 9p21-p12

Cellular Component: guanylate cyclase complex, soluble; integral to membrane; plasma membrane

Molecular Function: adenylate cyclase activity; ATP binding; GTP binding; guanylate cyclase activity; hormone binding; identical protein binding; natriuretic peptide receptor activity; peptide hormone binding; protein kinase activity; receptor activity; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; cGMP biosynthetic process; negative regulation of meiotic cell cycle; ossification; protein amino acid phosphorylation; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; signal transduction

Disease: Acromesomelic Dysplasia, Maroteaux Type; Epiphyseal Chondrodysplasia, Miura Type; Short Stature With Nonspecific Skeletal Abnormalities

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