Full Product Name
GDF6 siRNA (Mouse)
Product Synonym Names
BMP13; GDF-6; GDF16; Growth/differentiation factor 6; GDF-6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16
Product Gene Name
GDF6 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43028
Specificity
GDF6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GDF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GDF6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GDF6 sirna
siRNA to inhibit GDF6 expression using RNA interference
Applications Tested/Suitable for GDF6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GDF6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038554.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013526.1
[Other Products]
UniProt Primary Accession #
P43028
[Other Products]
UniProt Secondary Accession #
Q70UT4[Other Products]
UniProt Related Accession #
P43028[Other Products]
Molecular Weight
50,942 Da
NCBI Official Full Name
growth/differentiation factor 6
NCBI Official Synonym Full Names
growth differentiation factor 6
NCBI Official Symbol
Gdf6??[Similar Products]
NCBI Official Synonym Symbols
BMP13; GDF16; BMP-13
??[Similar Products]
NCBI Protein Information
growth/differentiation factor 6
UniProt Protein Name
Growth/differentiation factor 6
UniProt Synonym Protein Names
Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16
Protein Family
Growth/differentiation factor
UniProt Gene Name
Gdf6??[Similar Products]
UniProt Synonym Gene Names
Bmp13; Gdf-6; Gdf16; GDF-6; BMP-13??[Similar Products]
UniProt Entry Name
GDF6_MOUSE
UniProt Comments for GDF6
GDF6: Required for normal formation of bones and joints in the limbs, skull, and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Defects in GDF6 are the cause of Klippel-Feil syndrome type 1 (KFS1). A skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Deafness is a well- known feature of KFS and may be of sensorineural, conductive, or mixed type. A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3). Defects in GDF6 are the cause of microphthalmia isolated type 4 (MCOP4). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.
Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cytokine
Cellular Component: extracellular space; extracellular region
Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: regulation of apoptosis; BMP signaling pathway; apoptosis; regulation of MAPKKK cascade; multicellular organismal development; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; positive regulation of neuron differentiation; growth
Disease: Coloboma, Ocular, Autosomal Dominant
Research Articles on GDF6
1. Over-expression of Gdf6 induces commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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