Product Name
NOP10, siRNA
Full Product Name
NOP10 siRNA (Human)
Product Synonym Names
NOLA3; H/ACA ribonucleoprotein complex subunit 3; Nucleolar protein 10; Nucleolar protein family A member 3; snoRNP protein NOP10
Product Gene Name
NOP10 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NPE3
Specificity
NOP10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NOP10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NOP10 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NOP10 sirna
siRNA to inhibit NOP10 expression using RNA interference
Applications Tested/Suitable for NOP10 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NOP10. It may not necessarily be applicable to this product.
NCBI Accession #
NP_061118.1
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NCBI GenBank Nucleotide #
NM_018648.3
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UniProt Primary Accession #
Q9NPE3
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UniProt Related Accession #
Q9NPE3[Other Products]
Molecular Weight
7,706 Da
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit 3
NCBI Official Synonym Full Names
NOP10 ribonucleoprotein
NCBI Official Symbol
NOP10??[Similar Products]
NCBI Official Synonym Symbols
DKCB1; NOLA3; NOP10P
??[Similar Products]
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit 3
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 3
UniProt Synonym Protein Names
Nucleolar protein 10; Nucleolar protein family A member 3; snoRNP protein NOP10
Protein Family
Ribosome biogenesis protein
UniProt Gene Name
NOP10??[Similar Products]
UniProt Synonym Gene Names
NOLA3??[Similar Products]
UniProt Entry Name
NOP10_HUMAN
NCBI Summary for NOP10
This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA2 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nop10p. [provided by RefSeq, Jul 2008]
UniProt Comments for NOP10
NOP10: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in NOP10 are a cause of dyskeratosis congenita autosomal recessive type 1 (DKCB1). A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Belongs to the NOP10 family.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 15q14-q15
Cellular Component: Cajal body; small nucleolar ribonucleoprotein complex; nucleolus
Molecular Function: snoRNA binding; protein binding
Biological Process: pseudouridine synthesis; rRNA processing
Disease: Dyskeratosis Congenita, Autosomal Recessive, 1
Research Articles on NOP10
1. Indian aplastic anemia patients did not have NOP10 mutations.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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