Product Name
5'-AMP-activated protein kinase subunit beta-1 (Prkab1), Recombinant Protein
Full Product Name
Recombinant Mouse 5'-AMP-activated protein kinase subunit beta-1 (Prkab1)
Product Gene Name
Prkab1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Prkab1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-270aa, Full Length of Mature Protein
Sequence
GNTSSERAAL ERQAGHKTPR RDSSGGAKDG DRPKILMDSP EDADIFHSEE IKAPEKEEFL AWQHDLEAND KAPAQARPTV FRWTGGGKEV YLSGSFNNWS KLPLTRSQNN FVAILDLPEG EHQYKFFVDG QWTHDPSEPI VTSQLGTVNN IIQVKKTDFE VFDALMVDSQ KCSDVSELSS SPPGPYHQEP YMSKPEERFK APPILPPHLL QVILNKDTGI SCDPALLPEP NHVMLNHLYA LSIKDGVMVL SATHRYKKKY VTTLLYKPI
3D Structure
ModBase 3D Structure for Q9R078
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Prkab1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Prkab1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_114075.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031869.2
[Other Products]
UniProt Primary Accession #
Q9R078
[Other Products]
UniProt Secondary Accession #
Q91YN6[Other Products]
UniProt Related Accession #
Q9R078[Other Products]
Molecular Weight
30,308 Da
NCBI Official Full Name
5'-AMP-activated protein kinase subunit beta-1
NCBI Official Synonym Full Names
protein kinase, AMP-activated, beta 1 non-catalytic subunit
NCBI Official Symbol
Prkab1??[Similar Products]
NCBI Official Synonym Symbols
AU021155; E430008F22; 1300015D22Rik
??[Similar Products]
NCBI Protein Information
5'-AMP-activated protein kinase subunit beta-1
UniProt Protein Name
5'-AMP-activated protein kinase subunit beta-1
Protein Family
5'-AMP-activated protein kinase
UniProt Gene Name
Prkab1??[Similar Products]
UniProt Synonym Gene Names
AMPK subunit beta-1; AMPKb??[Similar Products]
UniProt Comments for Prkab1
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) ().
Research Articles on Prkab1
1. In lipid-laden macrophages, Ampk activation decreased cholesterol content (foam cell formation) and increased cholesterol efflux to HDL and apoA-I, effects that occurred in an Ampk beta1-dependent manner.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
