NLR family CARD domain-containing protein 4; CARD; LRR; and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf; NLRC4; CARD12; CLAN; CLAN1; IPAF

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This NLRC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the N-terminal region of human NLRC4.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-NLRC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

In C. elegans, Ced4 binds and activates Ced3, an apoptotic
initiator caspase, via caspase-associated recruitment domains
(CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4
(MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at
least 1 N-terminal CARD domain followed by a centrally located
nucleotide-binding domain (NBD or NACHT) and a C-terminal
regulatory domain, found only in mammals, that contains either WD40
repeats or leucine-rich repeats (LRRs). CARD12 is a member of the
Ced4 family and can induce apoptosis.

Cell Biology; Immunology; Metabolism

Western Blot (WB), ELISA (EIA)

WB~~1:1000

NLRC4 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NLRC4 antibody detected the NLRC4 protein (arrow).

116159

NLR family, CARD domain containing 4

NLR family CARD domain-containing protein 4

NLR family CARD domain-containing protein 4

CARD12; CLAN; CLAN1; IPAF; Clan protein; Ipaf??[Similar Products]

NLRC4_HUMAN

This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: spindle microtubule; cytoplasm; intracellular; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; ubiquitin-protein ligase activity; magnesium ion binding; cysteine protease inhibitor activity; ATP binding

Biological Process: caspase activation; detection of bacterium; positive regulation of apoptosis; protein ubiquitination; regulation of signal transduction; activation of NF-kappaB transcription factor; defense response to bacterium; regulation of neuron apoptosis; innate immune response; interleukin-1 beta secretion; inflammatory response; activation of innate immune response; protein homooligomerization; negative regulation of apoptosis

Disease: Autoinflammation With Infantile Enterocolitis; Familial Cold Autoinflammatory Syndrome 4

Miao, E.A., et al. J. Clin. Immunol. 30(4):502-506(2010)
Vinzing, M., et al. J. Immunol. 180(10):6808-6815(2008)
Macaluso, F., et al. Exp. Dermatol. 16(8):692-698(2007)
Bruey, J.M., et al. Cell 129(1):45-56(2007)
Thalappilly, S., et al. FEBS J. 273(12):2766-2778(2006)

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