Product Name
NLRC4, Polyclonal Antibody
Popular Item
Full Product Name
NLRC4 Polyclonal Antibody
Product Synonym Names
AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
Product Gene Name
anti-NLRC4 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
LSYQVTEEDL NVLAQNLKDL YNSPAFLNFY PLGEDIDIIF NLEKTFTEPI MWKKDHRHHR VEQLTLGSLL E
3D Structure
ModBase 3D Structure for Q9NPP4
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of mouse NLRC4
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, cytosol
Positive Samples
A-549, U-87MG, 293T, Mouse spleen
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-NLRC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NLRC4 antibody
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-NLRC4 antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-NLRC4 antibody
Western Blot (WB)
Application Notes for anti-NLRC4 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-NLRC4 antibody
Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
NCBI/Uniprot data below describe general gene information for NLRC4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001289433.1
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NCBI GenBank Nucleotide #
NM_001302504.1
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UniProt Primary Accession #
Q9NPP4
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UniProt Secondary Accession #
Q96J81; Q96J82; Q96J83; A8K9F8; B2RBQ3; B3KTF0; D6W580[Other Products]
UniProt Related Accession #
Q9NPP4[Other Products]
Molecular Weight
Calculated: 116kDa
Observed: 116kDa
NCBI Official Full Name
NLR family CARD domain-containing protein 4 isoform b
NCBI Official Synonym Full Names
NLR family CARD domain containing 4
NCBI Official Symbol
NLRC4??[Similar Products]
NCBI Official Synonym Symbols
CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
??[Similar Products]
NCBI Protein Information
NLR family CARD domain-containing protein 4
UniProt Protein Name
NLR family CARD domain-containing protein 4
UniProt Synonym Protein Names
CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf
Protein Family
NLR family CARD domain-containing protein
UniProt Gene Name
NLRC4??[Similar Products]
UniProt Synonym Gene Names
CARD12; CLAN; CLAN1; IPAF; Clan protein; Ipaf??[Similar Products]
NCBI Summary for NLRC4
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
UniProt Comments for NLRC4
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.
Research Articles on NLRC4
1. mRNA expression levels of NLRP1 and NLRC4 were not altered in chronic hepatitis B patients, suggesting that these genes are not responsible for the impaired immune responses against HBV observed in these patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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