Product Name
5'-Nucleotidase (NT5E), Polyclonal Antibody
Popular Item
Full Product Name
Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)
Product Gene Name
anti-NT5E antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
Immunogen: 5'-Nucleotidase (MBS2030180)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
APC-CY7 Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053130)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
PE Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053131)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
APC Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053132)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
Cy3 Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053133)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
FITC Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053134)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
HRP Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2053135)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: 5'-Nucleotidase (MBS2027940)
Biotin Conjugated Antibody: 5'-Nucleotidase, Ecto (NT5E) (MBS2093052)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SSK CVNASRCVGG VARLATKVHQ IRRAEPHVLL LDAGDQYQGT IWFTVYKGTE VAHFMNALGY DAMALGNHEF DNGVEGLIDP LLKEVNFPIL SANIKAKGPL ASKISGLYSP YKILTVGDEV VGIVGYTSKE TPFLSNPGTN LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEVDKLIA QKVKGVDVVV GGHSNTFLYT GNPPSKEVPA GQYPFIVTSD DGRKVPVVQA YAFGKYLGYL KVEFDEKGNV VTSHGNPILL NSSIPEDPNI KADINKWRVK LDNYSTQELG KTIVYLDGTA QSCRFRECNM GNLICDAMIN NNLRHPDEMS WNHVSMCILN GGGIRSPIDE RNNGTITWEN LAAVLPFGGT FDLVQLKGST LKKAFEHSVH RYGQATGEFL QVGGIHVVYD ISRNPGDRVV KLEVLCTQCR
3D Structure
ModBase 3D Structure for Q05927
Species Reactivity
Bovine
Specificity
The antibody is a rabbit polyclonal antibody raised against NT5E. It has beenselected for its ability to recognize NT5E in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Concentration
200ug/ml (lot specific)
Fragment
NT5E (Ser48~Val527)
Organism Species
Bos taurus; Bovine (Cattle)
Immunogen
Recombinant NT5E (Ser48~Val527) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2053132
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-NT5E antibody
Knock-Out Validation Antibody
Applications Tested/Suitable for anti-NT5E antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-NT5E antibody
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Western Blot (WB) of anti-NT5E antibody
Western Blot: Sample: Bovine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine NT5E Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot (WB) of anti-NT5E antibody
Western Blot: Sample: Recombinant protein.
Knockout Validation of anti-NT5E antibody
Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: NT5E knockout K562 cell lysate; Predicted MW: 63kd Observed MW: 70kd Primary Ab: 3ug/ml Rabbit Anti-Bovine NT5E Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.
NCBI Accession #
NP_776554.2
[Other Products]
NCBI GenBank Nucleotide #
NM_174129.3
[Other Products]
UniProt Primary Accession #
Q05927
[Other Products]
UniProt Secondary Accession #
Q24K05[Other Products]
UniProt Related Accession #
Q05927[Other Products]
Molecular Weight
62,966 Da
NCBI Official Full Name
5'-nucleotidase
NCBI Official Symbol
NT5E??[Similar Products]
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
Protein Family
5'-nucleotidase
UniProt Gene Name
NT5E??[Similar Products]
UniProt Synonym Gene Names
NT5; NTE; 5'-NT??[Similar Products]
UniProt Comments for NT5E
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
