Product Name
5'-nucleotidase (NT5), Recombinant Protein
Full Product Name
Recombinant Human 5'-nucleotidase (NT5), partial
Product Synonym Names
Ecto-5'-nucleotidase
Product Gene Name
NT5 recombinant protein
[Similar Products]
Product Synonym Gene Name
CD73[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
28-547aa
Sequence
ELTILHTNDV HSRLEQTSED SSKCVNASRC MGGVARLFTK VQQIRRAEPN VLLLDAGDQY QGTIWFTVYK GAEVAHFMNA LRYDAMALGN HEFDNGVEGL IEPLLKEAKF PILSANIKAK GPLASQISGL YLPYKVLPVG DEVVGIVGYT SKETPFLSNP GTNLVFEDEI TALQPEVDKL KTLNVNKIIA LGHSGFEMDK LIAQKVRGVD VVVGGHSNTF LYTGNPPSKE VPAGKYPFIV TSDDGRKVPV VQAYAFGKYL GYLKIEFDER GNVISSHGNP ILLNSSIPED PSIKADINKW RIKLDNYSTQ ELGKTIVYLD GSSQSCRFRE CNMGNLICDA MINNNLRHTD EMFWNHVSMC ILNGGGIRSP IDERNNGTIT WENLAAVLPF GGTFDLVQLK GSTLKKAFEH SVHRYGQSTG EFLQVGGIHV VYDLSRKPGD RVVKLDVLCT KCRVPSYDPL KMDEVYKVIL PNFLANGGDG FQMIKDELLR HDSGDQDINV VSTYISKMKV IYPAVEGRIK
3D Structure
ModBase 3D Structure for P21589
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Organism
Homo sapiens (Human)
Tag Information
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.
The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of NT5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NT5 recombinant protein
Hydrolyzes Extracellular domain nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Product Categories/Family for NT5 recombinant protein
Cardiovascular
SDS-Page of NT5 recombinant protein
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for NT5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001191742.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001204813.1
[Other Products]
UniProt Primary Accession #
P21589
[Other Products]
UniProt Secondary Accession #
O75520; Q5W116; B3KQI8[Other Products]
UniProt Related Accession #
P21589[Other Products]
Molecular Weight
62.6 kDa
NCBI Official Full Name
5'-nucleotidase isoform 2 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E??[Similar Products]
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
??[Similar Products]
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
NT5E??[Similar Products]
UniProt Synonym Gene Names
NT5; NTE; 5'-NT??[Similar Products]
NCBI Summary for NT5
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Comments for NT5
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Product References and Citations for NT5 recombinant protein
Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y.Eur. J. Biochem. 191:563-569 (1990)
Research Articles on NT5
1. Data show that MiR-422a and its target CD73 are involved in early loco(regional) recurrence of head and neck squamous cell carcinoma (HNSCC) tumors and are targets for personalized medicine.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
