NT5E (CD73) Human; 5'-Nucleotidase Ecto (CD73) Human Recombinant; NT; eN; NT5; NTE; eNT; CD73; E5NT; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; NT5E; NT5E (CD73)

For Research Use Only. Not for use in diagnostic procedures.

E Coli

Greater than 90% as determined by SDS-PAGE.

NT5E (CD73) protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Sterile Filtered colorless solution.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Small volumes of NT5E recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: NT5E (CD73) Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 247amino acids (27-252 a.a) and having a molecular mass of 27kDa. NT5E (CD73) is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.??

RECOMBINANT & NATURAL PROTEINS; CD Antigens

57,949 Da

5'-nucleotidase, ecto (CD73)

5'-nucleotidase; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase

5'-nucleotidase

NT5; NTE; 5'-NT??[Similar Products]

5NTD_HUMAN

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor; EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

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