Product Name
Kallikrein 11 (KLK11), Recombinant Protein
Full Product Name
Kallikrein 11, Recombinant, Human (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)
Product Gene Name
KLK11 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (51525487..51531290, complement). Location: 19q13.33
3D Structure
ModBase 3D Structure for Q9UBX7
Purity/Purification
Molecular Biology Grade
95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a 0.2um filtered solution of 25mM Tris, 10mM CaCl2, 0.15M NaCl, pH 7.5 at the concentration of ~0.7 mg/ml.
Activity
Measured by its activity to cleave a colormetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405nm and the extinction coefficient of 13,260 M-1cm-1.5
Specific Activity
Measured with 100uM substrate and 100ng enzyme in 100ul of 50mM Tris, 1M NaCl, 10mM EDTA, pH 8.5, 0.1mM DTNB at RT, is 450pmoles/min/ug.
Activity can be inhibited by AEBSF, dichloroisocoumarin and aprotinin.
Endotoxin Level
1EU per 1ug of the protein as determined by the LAL method.
Preparation and Storage
-70 degree C
Other Notes
Small volumes of KLK11 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KLK11 recombinant protein
Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain
Product Categories/Family for KLK11 recombinant protein
Molecular Biology; MB-Kallikrein
NCBI/Uniprot data below describe general gene information for KLK11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161077.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001167605.1
[Other Products]
UniProt Primary Accession #
Q9UBX7
[Other Products]
UniProt Secondary Accession #
O75837; Q0WXX5; Q8IXD7; Q9NS65[Other Products]
UniProt Related Accession #
Q9UBX7[Other Products]
Molecular Weight
The purified, secreted rhKLK11 corresponds to the pro form with an N-terminal sequence of E19TRIIKGFE. By SDS-PAGE under reducing conditions, the apparent molecular mass of the pro form is approximately 40kD.[Similar Products]
NCBI Official Full Name
kallikrein-11 isoform 3
NCBI Official Synonym Full Names
kallikrein-related peptidase 11
NCBI Official Symbol
KLK11??[Similar Products]
NCBI Official Synonym Symbols
TLSP; PRSS20
??[Similar Products]
NCBI Protein Information
kallikrein-11; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease
UniProt Protein Name
Kallikrein-11
UniProt Synonym Protein Names
Hippostasin; Serine protease 20; Trypsin-like protease
Protein Family
Kallikrein
UniProt Gene Name
KLK11??[Similar Products]
UniProt Synonym Gene Names
PRSS20; TLSP; hK11??[Similar Products]
UniProt Entry Name
KLK11_HUMAN
NCBI Summary for KLK11
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009]
UniProt Comments for KLK11
KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.-
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: Golgi apparatus; extracellular space
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on KLK11
1. Human airway trypsin-like protease can stimulate mucin5AC hypersecretion through a PAR2-mediated signaling pathway in 16HBE cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
