Product Name
HSD17B4, siRNA
Full Product Name
HSD17B4 siRNA (Human)
Product Synonym Names
EDH17B4; Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2
Product Gene Name
HSD17B4 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51659
Specificity
HSD17B4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HSD17B4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HSD17B4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSD17B4 sirna
siRNA to inhibit HSD17B4 expression using RNA interference
Applications Tested/Suitable for HSD17B4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HSD17B4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000405.1
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NCBI GenBank Nucleotide #
NM_000414.3
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UniProt Primary Accession #
P51659
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UniProt Secondary Accession #
B4DNV1; B4DVS5; E9PB82; F5HE57[Other Products]
UniProt Related Accession #
P51659[Other Products]
Molecular Weight
77,870 Da
NCBI Official Full Name
peroxisomal multifunctional enzyme type 2 isoform 2
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 4
NCBI Official Symbol
HSD17B4??[Similar Products]
NCBI Official Synonym Symbols
DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
??[Similar Products]
NCBI Protein Information
peroxisomal multifunctional enzyme type 2
UniProt Protein Name
Peroxisomal multifunctional enzyme type 2
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4
UniProt Gene Name
HSD17B4??[Similar Products]
UniProt Synonym Gene Names
EDH17B4; SDR8C1; MFE-2; 17-beta-HSD 4; DBP; MPF-2??[Similar Products]
UniProt Entry Name
DHB4_HUMAN
NCBI Summary for HSD17B4
The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for HSD17B4
HSD17B4: Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD). DBPD is a disorder of peroxisomal fatty acid beta-oxidation. Defects in HSD17B4 are the cause of Perrault syndrome (PRS). PRS is a sex-influenced disorder characterized by sensorineural deafness in both males and females and ovarian dysgenesis in females. Some patients also have neurologic manifestations, including mild mental retardation and cerebellar and peripheral nervous system involvement. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Lyase; Oxidoreductase; EC 1.1.1.n12; Mitochondrial; Cell development/differentiation; Lipid Metabolism - primary bile acid biosynthesis; EC 4.2.1.119; EC 4.2.1.107
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: peroxisomal membrane; peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; membrane; peroxisome
Molecular Function: protein homodimerization activity; 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; long-chain-enoyl-CoA hydratase activity; isomerase activity; 3-hydroxyacyl-CoA dehydrogenase activity; receptor binding
Biological Process: osteoblast differentiation; bile acid biosynthetic process; estrogen metabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid beta-oxidation; very-long-chain fatty acid metabolic process; metabolic process; bile acid metabolic process; unsaturated fatty acid metabolic process; androgen metabolic process; cellular lipid metabolic process; Sertoli cell development
Disease: Perrault Syndrome 1; D-bifunctional Protein Deficiency
Research Articles on HSD17B4
1. Structural MFE-2 instability is the molecular basis of D-bifunctional protein deficiency type III.
Precautions
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Disclaimer
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