For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HMGCS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS732559 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3 Hydroxy 3 methylglutaryl Coenzyme A Synthase (HMGCS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HMGCS. The ELISA analytical biochemical technique of the MBS732559 kit is based on HMGCS antibody-HMGCS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMGCS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMGCS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This HK3 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHK3 in the sample, thisHK3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHK3 concentration. The concentration ofHK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve

Principle of the Assay: This HK3 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHK3. Standards or samples are then added to the microtiter plate wells andHK3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHK3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHK3 are added to each well to "sandwich" theHK3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHK3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

57,294 Da

3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)

hydroxymethylglutaryl-CoA synthase, cytoplasmic; 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

HMGCS; HMG-CoA synthase??[Similar Products]

HMCS1_HUMAN

HMGCS1: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Belongs to the HMG-CoA synthase family.

Protein type: Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; EC 2.3.3.10; Transferase; Lipid Metabolism - synthesis and degradation of ketone bodies

Chromosomal Location of Human Ortholog: 5p14-p13

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein homodimerization activity; organic acid binding; hydroxymethylglutaryl-CoA synthase activity; isomerase activity; drug binding

Biological Process: response to drug; response to purine; response to vitamin E; isoprenoid biosynthetic process; response to tellurium ion; male gonad development; response to lipoprotein stimulus; lipid metabolic process; brain development; cellular lipid metabolic process; liver development; cholesterol biosynthetic process; response to low light intensity

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