Full Product Name
BARK1 (ADRBK1, GRK2) Antibody
Product Synonym Names
beta-ARK-1 G-protein coupled receptor kinase 2
Product Gene Name
anti-BARK1 antibody
[Similar Products]
Product Synonym Gene Name
GRK2; BARK1; BETA-ARK1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P25098
Species Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of ADRBK1(C-term.) and does not cross-react with related proteins.
Purity/Purification
Affinity purified
Form/Format
Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Immunogen
Purified recombinant human ADRBK1(C-term.) protein fragments expressed in E Coli.
Other Notes
Small volumes of anti-BARK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BARK1 antibody
UniPort Summary Function: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Entrez Summary: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-BARK1 antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-BARK1 antibody
Western Blot (WB)
Testing Data of anti-BARK1 antibody
Western blot detection of ADRBK1 in U937,Raw264.7&Ramos whole cell lysate(1:1000 diluted) Predicted band size:80KDa. Observed band size:80KDa.
NCBI/Uniprot data below describe general gene information for BARK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001610.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001619
[Other Products]
UniProt Primary Accession #
P25098
[Other Products]
UniProt Secondary Accession #
Q13837; Q6GTT3; B0ZBE1[Other Products]
UniProt Related Accession #
P25098[Other Products]
NCBI Official Full Name
beta-adrenergic receptor kinase 1
NCBI Official Synonym Full Names
G protein-coupled receptor kinase 2
NCBI Official Symbol
GRK2??[Similar Products]
NCBI Official Synonym Symbols
BARK1; ADRBK1; BETA-ARK1
??[Similar Products]
NCBI Protein Information
beta-adrenergic receptor kinase 1
UniProt Protein Name
Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names
G-protein coupled receptor kinase 2
UniProt Gene Name
GRK2??[Similar Products]
UniProt Synonym Gene Names
Beta-ARK-1??[Similar Products]
NCBI Summary for BARK1
This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]
UniProt Comments for BARK1
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925, PubMed:19715378). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity ().
Research Articles on BARK1
1. A novel regulatory role of GRK2 was proposed for the ubiquitination of beta-arrestin in the context of the PKC-mediated heterologous regulation of GPCRs.
Precautions
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