For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

IK22/5

Rat

This item recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases.

It is also expressed by T helper cells and augments the ability of dendritic cells to stimulate na?ve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses.

Clone IK22/5 blocks ligand binding.

Purified
Purified IgG - liquid

IgG concentration 0.5mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD254 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS219550 recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases. It is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naˉve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses. Clone IK22/5 blocks ligand binding.

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20

22,150 Da

tumor necrosis factor (ligand) superfamily, member 11

tumor necrosis factor ligand superfamily member 11; OPG ligand; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor-related activation-induced cytokine

Tumor necrosis factor ligand superfamily member 11

Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE??[Similar Products]

TNF11_MOUSE

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; cytoplasm; integral to membrane; plasma membrane; extracellular region; intracellular

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of osteoclast differentiation; multicellular organismal development; positive regulation of JNK cascade; osteoclast differentiation; activation of JNK activity; positive regulation of corticotropin-releasing hormone secretion; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; monocyte chemotaxis; positive regulation of MAP kinase activity; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; cell differentiation; protein homooligomerization; bone resorption; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; lymph node development; positive regulation of protein kinase B signaling cascade; organ morphogenesis; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of phosphorylation

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