Full Product Name
Anti-GRK2 Mouse mAb
Product Synonym Names
ADRBK 1; ADRBK1; Adrenergic beta receptor kinase 1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK 1; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G Protein Coupled Receptor Kinase 2; G protein dependent receptor kinase 2; G-protein coupled receptor kinase 2; GRK 2; GRK2
Product Gene Name
anti-GRK2 antibody
[Similar Products]
Product Synonym Gene Name
adrbk1; GRK2; BARK1; BETA-ARK1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P25098
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Concentration
2.5mg/ml (lot specific)
Immunogen
Purified recombinant human GRK2 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of GRK2 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
Subunit Structure
Interacts with GIT1 By similarity. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1.
Other Notes
Small volumes of anti-GRK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRK2 antibody
Entrez Summary: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-GRK2 antibody
Signal Transduction; Signaling Pathway; G Protein Signaling; GPCR;
Neuroscience; Neurotransmission; Receptors / Channels; GPCR; Adrenergic Receptors
Applications Tested/Suitable for anti-GRK2 antibody
Western Blot (WB)
Application Notes for anti-GRK2 antibody
WB: 1:500
Western Blot (WB) of anti-GRK2 antibody
Western blot detection of GRK2 in Ramos and THP-1 cell lysates using GRK2 mouse mAb (1:500 diluted), with super ECL.Predicted band size:80KDa.Observed band size:80KDa.Exposure time:20S.
NCBI/Uniprot data below describe general gene information for GRK2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001610.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001619
[Other Products]
UniProt Primary Accession #
P25098
[Other Products]
UniProt Secondary Accession #
Q13837; Q6GTT3; B0ZBE1[Other Products]
UniProt Related Accession #
P25098[Other Products]
NCBI Official Full Name
beta-adrenergic receptor kinase 1
NCBI Official Synonym Full Names
G protein-coupled receptor kinase 2
NCBI Official Symbol
GRK2??[Similar Products]
NCBI Official Synonym Symbols
BARK1; ADRBK1; BETA-ARK1
??[Similar Products]
NCBI Protein Information
beta-adrenergic receptor kinase 1
UniProt Protein Name
Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names
G-protein coupled receptor kinase 2
Protein Family
Beta-adrenergic receptor kinase
UniProt Gene Name
GRK2??[Similar Products]
UniProt Synonym Gene Names
Beta-ARK-1??[Similar Products]
NCBI Summary for GRK2
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
UniProt Comments for GRK2
GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
Protein type: AGC group; BARK subfamily; EC 2.7.11.15; GRK family; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: cytoplasm; cytosol; membrane; plasma membrane
Molecular Function: alpha-2A adrenergic receptor binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity
Biological Process: acetylcholine receptor signaling, muscarinic pathway; cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; peptidyl-serine phosphorylation; positive regulation of catecholamine secretion; receptor internalization; tachykinin signaling pathway; viral genome replication
Research Articles on GRK2
1. GRK2 may inhibit IGF1-induced human hepatocellular carcinoma cell growth and migration through downregulation of EGR1.
Precautions
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