ADRBK1; ARBK1; BARK; BARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; G protein receptor kinase 2; G- protein coupled receptor kinase 2; kinase GRK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

GRK2 (Phospho-Ser29) Antibody detects endogenous levels of GRK2 only when phosphorylated at Ser29.

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml (lot specific)

Stable at -20 degree C for at least 1 year.

Small volumes of anti-GRK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRK2 (Phospho-Ser29) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using GRK2 (Phospho-Ser29) Antibody. The lane on the right is blocked with the phospho peptide.

79 kDa

G protein-coupled receptor kinase 2

beta-adrenergic receptor kinase 1

Beta-adrenergic receptor kinase 1

Beta-ARK-1??[Similar Products]

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.

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