Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ; fast twitch skeletal muscle isoform; AT2A1_HUMAN; ATP2A; ATP2A1; ATPase Ca++ transporting cardiac muscle fast twitch 1; ATPase Ca++ transporting fast twitch 1; ATPase, Ca(2+)-transporting fast twitch 1; Calcium pump 1; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Calcium-transporting ATPase sarcoplasmic reticulum type; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Fast skeletal muscle SR calcium ATPase; OTTHUMP00000162561; OTTHUMP00000162562; Sarcoendoplasmic reticulum calcium ATPase; Sarcoplasmic reticulum Ca(2+)-ATPase 1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA 1; SERCA1; SERCA1 truncated isoform, included; SR Ca(2+) ATPase 1; SR Ca(2+)-ATPase 1; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Immunogen Affinity Purified

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ATP2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: SERCA1, also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped to 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. It has been determined that the human SERCA1 gene is 26 kb long and contains 23 exons, of which can be alternatively spliced. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Anti- SERCA1 ATPase antibody, Western blotting
All lanes: Anti SERCA1 ATPase at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 110KD
Observed bind size: 110KD

Anti- SERCA1 ATPase antibody,IHC(P)
IHC(P): Rat Skeletal Muscle Tissue

Anti- SERCA1 ATPase antibody, IHC(P)
IHC(P): Mouse Skeletal Muscle Tissue

95,199 Da

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1

sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

SERCA1; SR Ca(2+)-ATPase 1??[Similar Products]

AT2A1_HUMAN

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]

ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.8; Endoplasmic reticulum; Hydrolase; Transporter, ion channel; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment; I band; integral to membrane; integral to plasma membrane; membrane; mitochondrion; perinuclear region of cytoplasm; sarcoplasmic reticulum; sarcoplasmic reticulum membrane

Molecular Function: ATP binding; calcium ion binding; calcium-transporting ATPase activity; protein binding; protein homodimerization activity

Biological Process: apoptotic mitochondrial changes; calcium ion transport; elevation of endoplasmic reticulum calcium ion concentration; elevation of mitochondrial calcium ion concentration; maintenance of mitochondrion localization; metabolic process; negative regulation of striated muscle contraction; positive regulation of fast-twitch skeletal muscle contraction; reduction of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction

Disease: Brody Myopathy

1. Brandl, C. J., Green, N. M., Korczak, B., MacLennan, D. H. Two Ca(2+) ATPase genes: homologies and mechanistic implications of deduced amino acid sequences. Cell 44: 597-607, 1986. 2. Karpati, G., Charuk, J., Carpenter, S., Jablecki, C., Holland, P. Myopathy caused by a deficiency of Ca(2+)-adenosine triphosphatase in sarcoplasmic reticulum (Brody's disease). Ann. Neurol. 20: 38-49, 1986. 3. Zhang, Y., Fujii, J., Phillips, M. S., Chen, H.-S., Karpati, G., Yee, W.-C., Schrank, B., Cornblath, D. R., Boyland, K. B., MacLennan, D. H. Characterization of cDNA and genomic DNA encoding SERCA1, the Ca(2+)-ATPase of human fast-twitch skeletal muscle sarcoplasmic reticulum, and its elimination as a candidate gene for Brody disease. Gemomics 30: 415-424, 1995.

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