Full Product Name
IBSP Antibody
Product Synonym Names
BSP; BNSP; SP-II; BSP-II
Product Gene Name
anti-IBSP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P21815
Specificity
The antibody detects endogenous levels of total IBSP protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1.4 mg/ml (lot specific)
Immunogen Description
Synthetic peptide corresponding to a region derived from internal residues of human integrin-binding sialoprotein
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-IBSP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IBSP antibody
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
Product Categories/Family for anti-IBSP antibody
Total protein Ab
Applications Tested/Suitable for anti-IBSP antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IBSP antibody
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Testing Data of anti-IBSP antibody
Gel: 8%SDS-PAGE Lysates (from left to right): Hela, K562 and hepG2 cell Amount of lysate: 40ug per lane Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds
Immunohistochemistry (IHC) of anti-IBSP antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/30.
NCBI/Uniprot data below describe general gene information for IBSP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004958.2
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NCBI GenBank Nucleotide #
NM_004967.3
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UniProt Primary Accession #
P21815
[Other Products]
UniProt Related Accession #
P21815[Other Products]
Molecular Weight
35,148 Da
NCBI Official Full Name
bone sialoprotein 2
NCBI Official Synonym Full Names
integrin-binding sialoprotein
NCBI Official Symbol
IBSP??[Similar Products]
NCBI Official Synonym Symbols
BSP; BNSP; SP-II; BSP-II
??[Similar Products]
NCBI Protein Information
bone sialoprotein 2
UniProt Protein Name
Bone sialoprotein 2
UniProt Synonym Protein Names
Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein
Protein Family
Bone sialoprotein
UniProt Gene Name
IBSP??[Similar Products]
UniProt Synonym Gene Names
BNSP; BSP II??[Similar Products]
UniProt Entry Name
SIAL_HUMAN
NCBI Summary for IBSP
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for IBSP
Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cell adhesion; Secreted
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: extracellular space; membrane; extracellular region
Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion
Research Articles on IBSP
1. oxidized low-density lipoprotein-induced expression dependent on Runx2 expression
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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