GRK2 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Purified IgG was prepared from whole serum by affinity chromatography.

Liquid; Phosphate Buffered Saline

Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

Small volumes of anti-GRK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit anti GRK2 antibody recognizes beta-adrenergic receptor kinase 1, also known as ADRBK1, BARK or BARK1. It belongs to the family of serine/threonine protein kinases called GPCR kinases (GRKs) that phosphorylate agonist occupied receptors (Pitcher et al. 1998). PKC-mediated phosphorylation of GRK2 increases activity toward its substrates (Krasel et al. 2001). GRK2 mediates ERK activation downstream of the EGFR by acting as a Rho-activated scaffold protein (Robinson and Pitcher 2013). As GRK2 can modulate several hallmarks of cancer by participation in many signaling pathways, it was suggested that it acts as an oncomodifier (Nogues et al. 2017).

Western Blot (WB)

Western blot analysis of HT-29 cell lysate and mouse spleen and brain tissue lysates using Rabbit anti GRK2 antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking

Western blot analysis of normal (control) and GRK2 knockout (KO) 293T cell lysates using Rabbit anti GRK2 antibody at a 1/500 dilution. 3% non-fat dry milk was used for blocking

G protein-coupled receptor kinase 2

beta-adrenergic receptor kinase 1

Beta-adrenergic receptor kinase 1

BARK; BARK1; GRK2; Beta-ARK-1??[Similar Products]

ARBK1_HUMAN

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; acetylcholine receptor signaling, muscarinic pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; innate immune response; cardiac muscle contraction

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.