Full Product Name
GRK2 Polyclonal Antibody
Product Synonym Names
Beta-adrenergic receptor kinase 1 (Beta-ARK-1) (EC 2.7.11.15) (G-protein coupled receptor kinase 2)
Product Gene Name
anti-GRK2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P25098
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/mL (lot specific)
Immunogen
Synthesized peptide derived from human GRK2
Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol pH 7.4.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GRK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRK2 antibody
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Applications Tested/Suitable for anti-GRK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-GRK2 antibody
WB: 1:500-2000
ELISA: 1:10000-20000
Western Blot (WB) of anti-GRK2 antibody
Western Blot analysis of Mouse liver using GRK2 Polyclonal Antibody at dilution of 1:1000.
NCBI/Uniprot data below describe general gene information for GRK2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001610.2
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NCBI GenBank Nucleotide #
NM_001619.4
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UniProt Primary Accession #
P25098
[Other Products]
UniProt Secondary Accession #
Q13837; Q6GTT3; B0ZBE1[Other Products]
UniProt Related Accession #
P25098[Other Products]
NCBI Official Full Name
beta-adrenergic receptor kinase 1
NCBI Official Synonym Full Names
G protein-coupled receptor kinase 2
NCBI Official Symbol
GRK2??[Similar Products]
NCBI Official Synonym Symbols
BARK1; ADRBK1; BETA-ARK1
??[Similar Products]
NCBI Protein Information
beta-adrenergic receptor kinase 1
UniProt Protein Name
Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names
G-protein coupled receptor kinase 2
Protein Family
Beta-adrenergic receptor kinase
UniProt Gene Name
GRK2??[Similar Products]
UniProt Synonym Gene Names
Beta-ARK-1??[Similar Products]
NCBI Summary for GRK2
This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]
UniProt Comments for GRK2
GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
Protein type: AGC group; BARK subfamily; EC 2.7.11.15; GRK family; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: cilium; cytoplasm; cytosol; membrane; plasma membrane
Molecular Function: alpha-2A adrenergic receptor binding; ATP binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity
Biological Process: cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; G-protein coupled acetylcholine receptor signaling pathway; G-protein coupled receptor signaling pathway; heart development; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of catecholamine secretion; receptor internalization; tachykinin signaling pathway; viral genome replication
Research Articles on GRK2
1. KHSV miR-K3 activates the GRK2/CXCR2/AKT axis inducing KSHV-induced angiogenesis and promoting KSHV latency.
Precautions
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Disclaimer
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