Product Name
ATP2A1, siRNA
Full Product Name
ATP2A1 siRNA (Rat)
Product Synonym Names
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type. fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Product Gene Name
ATP2A1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q64578
Specificity
ATP2A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ATP2A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP2A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP2A1 sirna
siRNA to inhibit ATP2A1 expression using RNA interference
Applications Tested/Suitable for ATP2A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP2A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_478120.1
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NCBI GenBank Nucleotide #
NM_058213.1
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UniProt Primary Accession #
Q64578
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UniProt Related Accession #
Q64578[Other Products]
Molecular Weight
109,409 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 1
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
NCBI Official Symbol
Atp2a1??[Similar Products]
NCBI Official Synonym Symbols
Serca1
??[Similar Products]
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Synonym Protein Names
Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Protein Family
Sarcoplasmic/endoplasmic reticulum calcium ATPase
UniProt Gene Name
Atp2a1??[Similar Products]
UniProt Synonym Gene Names
SERCA1; SR Ca(2+)-ATPase 1??[Similar Products]
UniProt Entry Name
AT2A1_RAT
NCBI Summary for ATP2A1
catalyzes ATP hydrolysis and calcium ion transport [RGD, Feb 2006]
UniProt Comments for ATP2A1
ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ion channel; EC 3.6.3.8; Endoplasmic reticulum; Hydrolase
Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; metal ion binding; nucleotide-sugar transmembrane transporter activity; calcium ion binding; ATP binding
Biological Process: response to peptide hormone stimulus; apoptotic mitochondrial changes; metabolic process; positive regulation of fast-twitch skeletal muscle contraction; regulation of muscle contraction; maintenance of mitochondrion localization; calcium ion transport; elevation of endoplasmic reticulum calcium ion concentration; reduction of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction; negative regulation of striated muscle contraction; elevation of mitochondrial calcium ion concentration
Research Articles on ATP2A1
1. Compared with spinal isolation, spasticity promoted parvalbumin recovery (ninefold increase) and a SERCA2-to-SERCA1 transformation (84% increase in the ratio of SERCA1 to SERCA2) in tail muscle.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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