Product Name
SAR1B, siRNA
Full Product Name
SAR1B siRNA (Human)
Product Synonym Names
SARA2; SARB; GTP-binding protein SAR1b; GTP-binding protein B; GTBPB
Product Gene Name
SAR1B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y6B6
Specificity
SAR1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SAR1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SAR1B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SAR1B sirna
siRNA to inhibit SAR1B expression using RNA interference
Applications Tested/Suitable for SAR1B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SAR1B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001028675.1
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NCBI GenBank Nucleotide #
NM_001033503.2
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UniProt Primary Accession #
Q9Y6B6
[Other Products]
UniProt Secondary Accession #
Q567T4; D3DQA4[Other Products]
UniProt Related Accession #
Q9Y6B6[Other Products]
Molecular Weight
22,410 Da
NCBI Official Full Name
GTP-binding protein SAR1b
NCBI Official Synonym Full Names
secretion associated, Ras related GTPase 1B
NCBI Official Symbol
SAR1B??[Similar Products]
NCBI Official Synonym Symbols
ANDD; CMRD; GTBPB; SARA2
??[Similar Products]
NCBI Protein Information
GTP-binding protein SAR1b
UniProt Protein Name
GTP-binding protein SAR1b
UniProt Synonym Protein Names
GTP-binding protein B; GTBPB
Protein Family
GTP-binding protein
UniProt Gene Name
SAR1B??[Similar Products]
UniProt Synonym Gene Names
SARA2; SARB; GTBPB??[Similar Products]
UniProt Entry Name
SAR1B_HUMAN
NCBI Summary for SAR1B
The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for SAR1B
SAR1B: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD); also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. Belongs to the small GTPase superfamily. SAR1 family.
Protein type: G protein; G protein, monomeric, SAR1; G protein, monomeric; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: endoplasmic reticulum membrane; cytosol
Molecular Function: GTPase activity; GTP binding; metal ion binding
Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Disease: Chylomicron Retention Disease
Research Articles on SAR1B
1. although Sar1A antagonizes the lipoprotein secretion-promoting activity of Sar1B, both isoforms modulate the expression of genes encoding cholesterol biosynthetic enzymes and the synthesis of cholesterol de novo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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