Anti-ATP2A1 / SERCA1 Antibody (clone 1B11) IHC-plus; ATP2A1; SERCA1; ATP2A; Calcium pump 1; SR Ca(2+)-ATPase 1; Human ATP2A1; SERCA1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1,k

1B11

Mouse

Protein A Purified

PBS, pH 7.4.

0.5 mg/ml (lot specific)

Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Small volumes of anti-ATP2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Subfamily:-ATPase---P-type,-type-IIA">Family: Transporter
Subfamily: ATPase - P type, type IIA

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

ELISA (2.5 ug/ml), IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control A-431. Western Blot, use antibody at 1:500 to 1:1000 dilution (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control A-431 cells. ELISA - Re...

Anti-SERCA1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-SERCA1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of monoclonal antibody to ATP2A1 on A-431 cell (antibody concentration 10 ug/ml).

Western blot of ATP2A1 expression in transfected 293T cell line by ATP2A1 monoclonal antibody, clone 1B11.

ATP2A1 monoclonal antibody, clone 1B11 Western blot of ATP2A1 expression in A-431.

95,199 Da

ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

SERCA1; SR Ca(2+)-ATPase 1??[Similar Products]

AT2A1_HUMAN

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]

ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; Endoplasmic reticulum; EC 3.6.3.8; Hydrolase; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; ER-Golgi intermediate compartment; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; metabolic process; maintenance of mitochondrion localization; positive regulation of fast-twitch skeletal muscle contraction; reduction of endoplasmic reticulum calcium ion concentration; calcium ion transport; elevation of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction; blood coagulation; transmembrane transport; elevation of mitochondrial calcium ion concentration; negative regulation of striated muscle contraction

Disease: Brody Myopathy

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