Product Name
B3GALTL, Polyclonal Antibody
Popular Item
Full Product Name
B3GALTL Antibody
Product Synonym Names
B3GlcT; B3GTL; Beta 1, 3 galactosyltransferase like; Beta 3 galactosyltransferase like; Beta-1, 3-glucosyltransferase; Beta-3-glycosyltransferase-like; Beta3Glc-T; Beta3GlcT; Galactosyltransferase; GalT
Product Gene Name
anti-B3GALTL antibody
[Similar Products]
Product Synonym Gene Name
B3GLCT[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6Y288
Species Reactivity
Human, Mouse
Specificity
B3GALTL antibody detects endogenous levels of total B3GALTL
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Endoplasmic Reticulum
Tissue Specificity
Widely expressed, with highest levels in testis and uterus.
Predicted Cross Reactivity
Dog
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-B3GALTL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-B3GALTL antibody
Function: O-glucosyltransferase that transfers glucose toward fucose with a beta-1, 3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
Similarity: Belongs to the glycosyltransferase 31 family.
Applications Tested/Suitable for anti-B3GALTL antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-B3GALTL antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunohistochemistry (IHC) of anti-B3GALTL antibody
MBS9605291 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Western Blot (WB) of anti-B3GALTL antibody
Western blot analysis of extracts from HepG2 cells using B3GALTL antibody.The lane on the left is treated with the antigen-specific peptide.

Immunofluorescence (IF) of anti-B3GALTL antibody
MBS9605291 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

NCBI/Uniprot data below describe general gene information for B3GALTL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_919299.3
[Other Products]
NCBI GenBank Nucleotide #
NM_194318.3
[Other Products]
UniProt Primary Accession #
Q6Y288
[Other Products]
UniProt Secondary Accession #
Q5W0H2; Q6NUI3; A8K5F8[Other Products]
UniProt Related Accession #
Q6Y288[Other Products]
Molecular Weight
Observed: 57 kD
Predicted: 57 kDa
NCBI Official Full Name
beta-1,3-glucosyltransferase
NCBI Official Synonym Full Names
beta 3-glucosyltransferase
NCBI Official Symbol
B3GLCT??[Similar Products]
NCBI Official Synonym Symbols
B3GTL; Gal-T; B3GALTL; B3Glc-T; beta3Glc-T
??[Similar Products]
NCBI Protein Information
beta-1,3-glucosyltransferase
UniProt Protein Name
Beta-1,3-glucosyltransferase
UniProt Synonym Protein Names
Beta 3-glucosyltransferase
UniProt Gene Name
B3GLCT??[Similar Products]
UniProt Synonym Gene Names
B3GALTL; B3GTL; Beta3Glc-T??[Similar Products]
NCBI Summary for B3GALTL
The protein encoded by this gene is a beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins. The encoded protein is a type II membrane protein. Defects in this gene are a cause of Peters-plus syndrome (PPS).[provided by RefSeq, Mar 2009]
UniProt Comments for B3GALTL
O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
Research Articles on B3GALTL
1. Studies indicate that Peters Plus syndrome is caused by mutations in beta 3-glucosyltransferase (B3GALTL).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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