Full Product Name
Anti-HPRT Rabbit pAb
Product Gene Name
anti-HPRT antibody
[Similar Products]
Product Synonym Gene Name
HGPRT; HGPRTase; HPRT1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P00492
Species Reactivity
Human, Mouse, Rat
Specificity
HPRT Antibody detects endogenous levels of total HPRT
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human HPRT
Subcellular Location
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Other Notes
Small volumes of anti-HPRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HPRT antibody
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Applications Tested/Suitable for anti-HPRT antibody
Western Blot (WB), Immunhistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-HPRT antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
IP: 1:50
Western Blot (WB) of anti-HPRT antibody
Western blot analysis of HPRT expression in A549 cell lysate.
NCBI/Uniprot data below describe general gene information for HPRT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000185.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000194.2
[Other Products]
UniProt Primary Accession #
P00492
[Other Products]
UniProt Secondary Accession #
A6NHF0; B2R8M9[Other Products]
UniProt Related Accession #
P00492[Other Products]
NCBI Official Full Name
hypoxanthine-guanine phosphoribosyltransferase
NCBI Official Synonym Full Names
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol
HPRT1??[Similar Products]
NCBI Official Synonym Symbols
HPRT; HGPRT
??[Similar Products]
NCBI Protein Information
hypoxanthine-guanine phosphoribosyltransferase
UniProt Protein Name
Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name
HPRT1??[Similar Products]
UniProt Synonym Gene Names
HPRT; HGPRT; HGPRTase??[Similar Products]
NCBI Summary for HPRT
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
UniProt Comments for HPRT
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Research Articles on HPRT
1. mutation in the human HPRT1 gene and the Lesch-Nyhan disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
