Goat Anti-BMI1 (aa237-251) Antibody; BMI1; BMI1 polycomb ring finger oncogene; FLVI2/BMI1; PCGF4; RNF51; B lymphoma Mo-MLV insertion region 1 homolog; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; polycomb complex protein BMI-1; polycomb group RING finger protein 4; polycomb; BMI1 antibody; BMI1 polycomb ring finger oncogene antibody; FLVI2/BMI1 antibody; PCGF4 antibody; RNF51 antibody; B lymphoma Mo-MLV insertion region 1 homolog antibody; flvi-2/bmi-1 antibody; murine leukemia viral (bmi-1) oncogene homolog antibody; polycomb complex protein BMI-1 antibody; polycomb group RING finger protein 4 antibody; polycomb antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-BMI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 40kDa band observed in nuclear lysates of cell line K562 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev.2006 Aug 1;20(15): 2110-20. PMID: 16882984). Recommended concentration: 0. 03-0.1ug/ml.

(0. 03ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

36,949 Da

BMI1 proto-oncogene, polycomb ring finger

polycomb complex protein BMI-1

Polycomb complex protein BMI-1

PCGF4; RNF51??[Similar Products]

BMI1_HUMAN

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-; Oncoprotein

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: cytoplasm; nucleoplasm; nucleus; PcG protein complex; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding

Biological Process: chromatin modification; hemopoiesis; negative regulation of gene expression, epigenetic; negative regulation of transcription from RNA polymerase II promoter; positive regulation of fibroblast proliferation; positive regulation of ubiquitin-protein ligase activity; protein sumoylation; regulation of gene expression; segment specification; transcription, DNA-dependent

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