Product Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 (HSD3B2), Recombinant Protein
Full Product Name
Recombinant Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 (HSD3B2)
Product Gene Name
HSD3B2 recombinant protein
[Similar Products]
Product Synonym Gene Name
HSDB3B[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-372aa; Full length protein
Sequence
GWSCLVTGAG GLLGQRIVRL LVEEKELKEI RALDKAFRPE LREEFSKLQN RTKLTVLEGD ILDEPFLKRA CQDVSVVIHT ACIIDVFGVT HRESIMNVNV KGTQLLLEAC VQASVPVFIY TSSIEVAGPN SYKEIIQNGH EEEPLENTWP TPYPYSKKLA EKAVLAANGW NLKNGDTLYT CALRPTYIYG EGGPFLSASI NEALNNNGIL SSVGKFSTVN PVYVGNVAWA HILALRALRD PKKAPSVRGQ FYYISDDTPH QSYDNLNYIL SKEFGLRLDS RWSLPLTLMY WIGFLLEVVS FLLSPIYSYQ PPFNRHTVTL SNSVFTFSYK KAQRDLAYKP LYSWEEAKQK TVEWVGSLVD RHKETLKSKT Q
3D Structure
ModBase 3D Structure for P26439
Host
Cell Free Expression
Form/Format
Liquid containing glycerol
Storage Buffer
Tris-based buffer, 50% glycerol.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of HSD3B2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for HSD3B2 recombinant protein
Transmembrane Protein
Application Notes for HSD3B2 recombinant protein
This is a recombinant transmembrane protein expressed in a cell-free expression system.
NCBI/Uniprot data below describe general gene information for HSD3B2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000189.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000198.3
[Other Products]
UniProt Primary Accession #
P26439
[Other Products]
UniProt Secondary Accession #
Q16010; Q53GD4; Q6AI10; Q6LDB9; Q99890; Q9UD08; A2RRA5[Other Products]
UniProt Related Accession #
P26439[Other Products]
Molecular Weight
24,987 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
NCBI Official Symbol
HSD3B2??[Similar Products]
NCBI Official Synonym Symbols
HSDB; HSD3B; SDR11E2
??[Similar Products]
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal typeIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
Protein Family
4-isomerase" title="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase">3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
UniProt Gene Name
HSD3B2??[Similar Products]
UniProt Synonym Gene Names
HSDB3B; 3-beta-HSD II??[Similar Products]
UniProt Entry Name
3BHS2_HUMAN
NCBI Summary for HSD3B2
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for HSD3B2
HSD3B2: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.
Protein type: Lipid Metabolism - C21-steroid hormone; EC 1.1.1.145; Endoplasmic reticulum; Mitochondrial; Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; Isomerase; EC 5.3.3.1
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial membrane; smooth endoplasmic reticulum membrane
Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity
Biological Process: androgen biosynthetic process; glucocorticoid biosynthetic process; mineralocorticoid biosynthetic process; steroid biosynthetic process
Disease: 3-beta-hydroxysteroid Dehydrogenase, Type Ii, Deficiency Of
Research Articles on HSD3B2
1. HSD3B1 is involved in aldosterone production, despite its lower levels of expression compared with HSD3B2, and also possibly associated with KCNJ5 mutation in aldosterone-producing adenoma.
Precautions
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Disclaimer
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