Product Name
ATF7IP, siRNA
Full Product Name
ATF7IP siRNA (Mouse)
Product Synonym Names
MCAF1; Activating transcription factor 7-interacting protein 1; ATFa-associated modulator; mAM; MBD1-containing chromatin-associated factor 1
Product Gene Name
ATF7IP sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7TT18
Specificity
ATF7IP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATF7IP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATF7IP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATF7IP sirna
siRNA to inhibit ATF7IP expression using RNA interference
Applications Tested/Suitable for ATF7IP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATF7IP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062299.2
[Other Products]
NCBI GenBank Nucleotide #
NM_019426.2
[Other Products]
UniProt Primary Accession #
Q7TT18
[Other Products]
UniProt Secondary Accession #
Q3TJ86; Q3TZW1; Q3UVK5; Q3UYZ7; Q80ZK7; Q8C4A1; Q9JK31[Other Products]
UniProt Related Accession #
Q7TT18[Other Products]
Molecular Weight
37,258 Da
NCBI Official Full Name
activating transcription factor 7-interacting protein 1
NCBI Official Synonym Full Names
activating transcription factor 7 interacting protein
NCBI Official Symbol
Atf7ip??[Similar Products]
NCBI Official Synonym Symbols
AM; Mcaf1; 2610204M12Rik
??[Similar Products]
NCBI Protein Information
activating transcription factor 7-interacting protein 1
UniProt Protein Name
Activating transcription factor 7-interacting protein 1
UniProt Synonym Protein Names
ATFa-associated modulator; mAM; MBD1-containing chromatin-associated factor 1
Protein Family
Activating transcription factor 7-interacting protein
UniProt Gene Name
Atf7ip??[Similar Products]
UniProt Synonym Gene Names
Mcaf1; mAM??[Similar Products]
UniProt Entry Name
MCAF1_MOUSE
UniProt Comments for ATF7IP
ATF7IP: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells. Belongs to the MCAF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: ATPase activity; transcription corepressor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of transcriptional preinitiation complex assembly
Research Articles on ATF7IP
1. We demonstrated the importance of Aire's interaction with the ATF7ip-MBD1 protein complex in maintaining central tolerance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
