Product Name
BLMH / Bleomycin Hydrolase, Recombinant Protein
Full Product Name
Recombinant Mouse BLMH / Bleomycin Hydrolase Protein (His tag)
Product Synonym Names
RP23-100P23.3, AI035728, Bh, Bmh, MGC36899, MGC37104, MGC390261
Product Gene Name
BLMH recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8R016
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of BLMH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BLMH recombinant protein
Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different type s of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
Description: A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.
Application Notes for BLMH recombinant protein
The recombinant mouse BLMH consisting of 461 amino acids and has a calculated molecular mass of 53.3 kDa. rmBLMH migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
SDS-PAGE of BLMH recombinant protein
NCBI/Uniprot data below describe general gene information for BLMH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_848760.1
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NCBI GenBank Nucleotide #
NM_178645.4
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UniProt Primary Accession #
Q8R016
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UniProt Secondary Accession #
Q3TJR8; Q8BLZ4; Q8BZH9; Q8C111; Q8CID9[Other Products]
UniProt Related Accession #
Q8R016[Other Products]
Molecular Weight
52,511 Da
NCBI Official Full Name
bleomycin hydrolase
NCBI Official Synonym Full Names
bleomycin hydrolase
NCBI Official Symbol
Blmh??[Similar Products]
NCBI Official Synonym Symbols
Bh; Bmh; AI035728
??[Similar Products]
NCBI Protein Information
bleomycin hydrolase
UniProt Protein Name
Bleomycin hydrolase
Protein Family
Bleomycin hydrolase
UniProt Gene Name
Blmh??[Similar Products]
UniProt Synonym Gene Names
BH; BLM hydrolase; BMH??[Similar Products]
NCBI Summary for BLMH
The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin, a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved, and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq, Jan 2010]
UniProt Comments for BLMH
BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.
Protein type: EC 3.4.22.40; Protease
Chromosomal Location of Human Ortholog: 11|11 B5
Cellular Component: cytoplasm; nucleus
Molecular Function: aminopeptidase activity; cysteine-type peptidase activity; peptidase activity
Biological Process: homocysteine catabolic process; response to drug; response to toxin
Research Articles on BLMH
1. Blmh interacts with diverse cellular processes--lipoprotein, amino acid and protein, carbohydrate, and energy metabolisms, detoxification, antioxidant defenses--that are essential for normal kidney homeostasis.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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