Mouse Programmed cell death 1 ligand 1 (CD274) ELISA kit; B7-H; B7H1; MGC142294; MGC142296; PD-L1; PDCD1L1; PDCD1LG1; PDL1; CD274 antigen; programmed cell death 1 ligand 1; CD274 molecule

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse CD274. No significant cross-reactivity or interference between mouse CD274 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CD274 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS940928 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD274 molecule, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD274. The ELISA analytical biochemical technique of the MBS940928 kit is based on CD274 antibody-CD274 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD274 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD274. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Programmed cell death ligand 1 PD L1 or CD274 molecule or B7 H1 has become the prominent player in the newest immunotherapies for cancer. No wonder our CD274 ELISA kit for mouse samples (or our human ELISA kits) it a top seller and performer. Each B7 H1 PD L1 molecule ELISA assay is faster - minutes to set up - will detect a wide range of proteins concentrations and is available as economical wells or plate formats. The reagents for CD274 B7 H1 sandwich ELISA kits include primary antibody, secondary antibodies, wash solution, detection reagent - TMB substrate - standard curve protein, sample diluent and stop solution. Lyse your cells or use TC supernatants, your results for cell death protein 1 will be reproducible and publishable the first time. MyBiosource.com offers many application details and specific target details for all our programmed death ligand products. Visit us today.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD274 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD274 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD274 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD274 bound in the initial step. The color development is stopped and the intensity of the color is measured.

32,780 Da

CD274 antigen

programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1

Programmed cell death 1 ligand 1

B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1??[Similar Products]

PD1L1_MOUSE

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction; positive regulation of cell migration

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