Polyclonal Anti- CD9

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 12; NC_000012.11 (6309482..6347437). Location: 12p13.3

Polyclonal

IgG

Rabbit

Immunogen affinity purified

Liquid

Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD9 antigen is a protein that in humans is encoded by the CD9 gene.1 CD9 was found to be intensely expressed on the cell surface of the glandular epithelium throughout the menstrual cycle without any apparent differences in staining intensity. In addition, Western blotting analysis of the affinity-purified proteins confirmed that CD9 was associated with integrins beta 1, alpha 3 and alpha 6 in the human endometrium. CD9 may be an important regulator of these integrins in the human endometrium.2 This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization, as CD9 knocked-out mice gametes don't undergo fusion.3 CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.4

Western Blot (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

WB (1:500-1000)
ICC (1:100)
Flow Cytometry (1:10-100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.

25,416 Da

CD9 molecule

CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein

CD9 antigen

MIC3; TSPAN29; MRP-1; Tspan-29??[Similar Products]

CD9_HUMAN

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Ref.11 Ref.12 Ref.13

Subunit structure: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Ref.14 Ref.16 Ref.17

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Expressed by a variety of hematopoietic and epithelial cells.

Post-translational modification: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

Sequence similarities: Belongs to the tetraspanin (TM4SF) family.

1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (Mar 1984). "Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells". Eur J Immunol 13 (12): 1008-1013.2. Park, K R; Inoue T, Ueda M, Hirano T, Higuchi T, Maeda M, Konishi I, Fujiwara H, Fujii S (Mar. 2000). "CD9 is expressed on human endometrial epithelial cells in association with integrins alpha(6), alpha(3) and beta(1)". Mol. Hum. Reprod. (ENGLAND) 6 (3): 252-257.3. Fran?ois Le Naour et al (2000). "Severely Reduced Female Fertility in CD9-Deficient Mice". Science 287 (5451): 319-321. 4. Kathryn E. Runge et al (2007). "Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution". Developmental Biology 304 (1): 317-325.

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