For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

MM2/57

Mouse

Purified
Purified IgG - liquid

IgG concentration 1.0 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD9 antibody, clone MM2/57 recognizes human Leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells. Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.

Immunohistology Frozen, Flow cytometry (FC/FACS), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Immunohistology - Frozen: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200

Staining of human peripheral blood platelets with Mouse anti Human CD9:RPE (MBS213834)

Staining of acetone fixed frozen human tonsil with Mouse anti Human CD9

Staining of human peripheral blood monocytes with Mouse anti Human CD9: Alexa Fluor 647

Staining of human peripheral blood platelets with Mouse anti Human CD9:FITC (MBS211024)

Staining of human peripheral blood platelets with Mouse anti Human CD9:Biotin (MBS210488)

25,416 Da

CD9 molecule

CD9 antigen; 5H9 antigen; BA-2/p24 antigen; CD9 antigen (p24); cell growth-inhibiting gene 2 protein; leukocyte antigen MIC3; motility related protein-1; tetraspanin-29

CD9 antigen

MIC3; TSPAN29; MRP-1; Tspan-29??[Similar Products]

CD9_HUMAN

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: platelet activation; fusion of sperm to egg plasma membrane; pathogenesis; multicellular organism reproduction; paranodal junction assembly; negative regulation of cell proliferation; platelet degranulation; single fertilization; brain development; cell adhesion; blood coagulation; cell motility; oligodendrocyte development; response to water deprivation

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