For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

MF1

Rat

Purified
Purified IgG - liquid

IgG concentration 1.0mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rat anti Mouse CD9 antibody, clone MF1 recognizes mouse CD9, a 25kD member of the tetraspanin family of transmembrane proteins. The tetraspanin family are multifunctional molecules which bind to a wide range of transmembrane proteins and intracellular signalling molecules. Tetraspanins facilitate the formation of molecular complexes which play a role in cell activation, proliferation, motility, fusion and apoptosis. CD9 is ubiquitously expressed, with high levels found on platelets and in male reproductive tissues. In mouse haematopoietic tissues, CD9 is expressed by myeloid and stromal cells. CD9 is known to complex with integrins and other transmembrane proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.

Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blot: This item detects a band of approximately 25kDa in cell lysates.

Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9:Endotoxin Low (MBS224726)

Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9 (MBS224489)

Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9 (MBS224547)

Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9:RPE(MBS224632)

25,258 Da

CD9 antigen

CD9 antigen

CD9 antigen

CD9_MOUSE

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass

Cellular Component: extracellular space; cell surface; focal adhesion; membrane; apical plasma membrane; plasma membrane; integral to membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: negative regulation of cell proliferation; fusion of sperm to egg plasma membrane; single fertilization; cell adhesion; cell motility; paranodal junction assembly

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