Mouse Semaphorin-3E (SEMA3E) ELISA kit; KIAA0331; M-SEMAH; M-SemaK; SEMAH; coll-5; semaphorin 3E; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3E

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse SEMA3E. No significant cross-reactivity or interference between mouse SEMA3E and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SEMA3E elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS937095 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA3E. The ELISA analytical biochemical technique of the MBS937095 kit is based on SEMA3E antibody-SEMA3E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA3E antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA3E. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3E is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3E bound in the initial step. The color development is stopped and the intensity of the color is measured.

89,543 Da

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E

semaphorin-3E; sema H; semaphorin-H

Semaphorin-3E

Semah; Semh; Sema H??[Similar Products]

SEM3E_MOUSE

SEMA3E: a secreted protein of the semaphorin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: membrane; extracellular region

Molecular Function: protein binding; receptor activity; semaphorin receptor binding

Biological Process: patterning of blood vessels; regulation of cell shape; nervous system development; negative regulation of angiogenesis; multicellular organismal development; synapse organization and biogenesis; angiogenesis; sprouting angiogenesis; negative regulation of cell-matrix adhesion; cell differentiation

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