Human Alpha 1-Antichymotrypsin

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_000014.8 (95078714..95090390). Location: 14q32.1

Human

Store at 4 degree C.

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Small volumes of SERPINA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS564032 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINA3. The ELISA analytical biochemical technique of the MBS564032 kit is based on SERPINA3 antibody-SERPINA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Alpha 1 antichymotrypsin AAT and antitypsin family are proteins highly expressed in human liver. They function as antiproteinase and peptidase inhibitor reagents to protect tissues from devastating cycles of fibrosis and inflammation. Serpin 3 protein mutations or loss are associated with fatal pediatric liver disease so experimental quantitation is a valuable tool. MyBiosource offers a leading human alpha antitrypsin member 3 ELISA kit for studies of disease, such as iPSC disease in a dish experiments. Shipped for rapid use with antibody bound plate, standard curve peptide, secondary antibodies, detection solution and ng ml dynamic range, our 1 antitrypsin ELISA kits are ideal for your sorption peptidase inhibitor drug screening campaigns. Assay your samples singly or in tandem arrays for maximal serpina results. Login to MyBiosource.com to view all proteins antibodies ELISA and inhibitor Clade products. Each invaluable in your research for the prefect (antiproteinase antitrypsin) serpin peptidase inhibitor.

Intended Uses: The Alpha 1-Antichymotrypsin (CYT) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1-Antichymotrypsin in biological fluids of humans.

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CYT present in samples reacts with the anti-CYT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CYT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CYT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CYT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CYT in the test sample. The quantity of CYT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA Kit

47,651 Da[Similar Products]

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3

alpha-1-antichymotrypsin; serpin A3; OTTHUMP00000028439; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3

Alpha-1-antichymotrypsin

AACT??[Similar Products]

AACT_HUMAN

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq]

SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space; extracellular region; intracellular; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; DNA binding

Biological Process: regulation of lipid metabolic process; maintenance of gastrointestinal epithelium; acute-phase response; inflammatory response

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