Product Name
bisphosphoglycerate mutase (BPGM), Polyclonal Antibody
Full Product Name
bisphosphoglycerate mutase antibody
Product Gene Name
anti-BPGM antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 216 of Human BPGM
Target Name
bisphosphoglycerate mutase
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-BPGM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-BPGM antibody
Total protein Ab
Applications Tested/Suitable for anti-BPGM antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-BPGM antibody
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Testing Data of anti-BPGM antibody
Sample(30 ug whole cell lysate)A: 293TB: A431C: H129912% SDS PAGEPrimary antibody diluted at 1: 1000
Immunofluorescence (IF) of anti-BPGM antibody
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM antibody at 1: 100 dilution.
NCBI/Uniprot data below describe general gene information for BPGM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001715
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NCBI GenBank Nucleotide #
NM_001724.4
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UniProt Secondary Accession #
A4D1N9[Other Products]
UniProt Related Accession #
P07738[Other Products]
Molecular Weight
30,005 Da[Similar Products]
NCBI Official Full Name
bisphosphoglycerate mutase
NCBI Official Synonym Full Names
2,3-bisphosphoglycerate mutase
NCBI Official Symbol
BPGM??[Similar Products]
NCBI Official Synonym Symbols
DPGM
??[Similar Products]
NCBI Protein Information
bisphosphoglycerate mutase
UniProt Protein Name
Bisphosphoglycerate mutase
UniProt Synonym Protein Names
2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11); 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM
Protein Family
Bisphosphoglycerate mutase
UniProt Gene Name
BPGM??[Similar Products]
UniProt Synonym Gene Names
BPGM; DPGM??[Similar Products]
UniProt Entry Name
PMGE_HUMAN
NCBI Summary for BPGM
2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
UniProt Comments for BPGM
BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
Protein type: Phosphatase (non-protein); EC 5.4.2.11; Isomerase; EC 5.4.2.4; EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Chromosomal Location of Human Ortholog: 7q33
Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity
Biological Process: erythrocyte development; glycolysis; dephosphorylation; carbohydrate metabolic process; respiratory gaseous exchange
Disease: Bisphosphoglycerate Mutase Deficiency
Research Articles on BPGM
1. 1.94 A resolution X-ray structure of bisphosphoglycerate mutase is presented, focusing on the dynamic nature of key ligand-binding residues and their interaction with the inhibitor citrate
Precautions
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Disclaimer
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