Full Product Name
GP5 siRNA (Human)
Product Synonym Names
Platelet glycoprotein V; GPV; Glycoprotein 5; CD antigen CD42d
Product Gene Name
GP5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P40197
Specificity
GP5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GP5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GP5 sirna
siRNA to inhibit GP5 expression using RNA interference
Applications Tested/Suitable for GP5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GP5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004479.1
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NCBI GenBank Nucleotide #
NM_004488.2
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UniProt Primary Accession #
P40197
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UniProt Secondary Accession #
D1MER9[Other Products]
UniProt Related Accession #
P40197[Other Products]
Molecular Weight
60,959 Da
NCBI Official Full Name
platelet glycoprotein V
NCBI Official Synonym Full Names
glycoprotein V (platelet)
NCBI Official Symbol
GP5??[Similar Products]
NCBI Official Synonym Symbols
GPV; CD42d
??[Similar Products]
NCBI Protein Information
platelet glycoprotein V
UniProt Protein Name
Platelet glycoprotein V
UniProt Synonym Protein Names
Glycoprotein 5; CD_antigen: CD42d
Protein Family
Glycoprotein
UniProt Gene Name
GP5??[Similar Products]
UniProt Synonym Gene Names
GPV??[Similar Products]
UniProt Entry Name
GPV_HUMAN
NCBI Summary for GP5
Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome (MIM 231200), a bleeding disorder (review by Lopez et al., 1998 [PubMed 9616133]).[supplied by OMIM, Nov 2010]
UniProt Comments for GP5
GPV: The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: collagen binding
Biological Process: platelet activation; cell-matrix adhesion; cell adhesion; blood coagulation; blood coagulation, intrinsic pathway
Research Articles on GP5
1. Studies indicate that platelets from Bernard-Soulier syndrome (BSS) are defective in glycoprotein (GP)Ib-IX-V, a platelet-specific adhesion-signaling complex, composed of GPIbalpha disulfide linked to GPIbbeta, and noncovalently associated with GPIX and GPV.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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