Cytochrome P450 1B1; Aryl hydrocarbon hydroxylase; CP1B; CP1B1_HUMAN; Cyp1b1; CYPIB1; Cytochrome P450 1B1; Cytochrome P450 family 1 subfamily B polypeptide 1; Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile); Flavoprotein linked monooxygenase; GLC3A; Microsomal monooxygenase; P4501B1; Xenobiotic monooxygenase; cytochrome P450, family 1, subfamily B, polypeptide 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Immunogen Affinity Purified

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-CYP1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Cytochrome P450 1B1(CYP1B1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Cytochrome P450 1B1 is an enzyme that in humans is encoded by the CYP1B1 gene. CYP1B1 belongs to the the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Anti- CYP1B1 Picoband antibody, MBS178206, Western blotting
All lanes: Anti CYP1B1 (MBS178206) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD

Anti- CYP1B1 Picoband antibody, MBS178206,IHC(P)
IHC(P): Mouse Kidney Tissue

Anti- CYP1B1 Picoband antibody, MBS178206,IHC(P)
IHC(P): Rat Kidney Tissue

Anti- CYP1B1 Picoband antibody, MBS178206,IHC(P)
IHC(P): Human Liver Cancer Tissue

60,846 Da

cytochrome P450 family 1 subfamily B member 1

cytochrome P450 1B1

Cytochrome P450 1B1

CP1B1_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]

CYP1B1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A). GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG). POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes. Defects in CYP1B1 are a cause of Peters anomaly (PAN). Peters anomaly is a congenital defect of the anterior chamber of the eye. Belongs to the cytochrome P450 family.

Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.14.14.1; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 2p22.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion

Molecular Function: heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: angiogenesis; arachidonic acid metabolic process; aromatic compound metabolic process; blood vessel morphogenesis; cell adhesion; collagen fibril organization; endothelial cell migration; epoxygenase P450 pathway; estrogen metabolic process; induction of apoptosis by oxidative stress; inhibition of NF-kappaB transcription factor; membrane lipid catabolic process; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of cell proliferation; nitric oxide biosynthetic process; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of JAK-STAT cascade; response to toxin; retinal metabolic process; retinol metabolic process; steroid metabolic process; sterol metabolic process; toxin metabolic process; visual perception; xenobiotic metabolic process

Disease: Glaucoma 3, Primary Congenital, A; Glaucoma 3, Primary Infantile, B; Peters Anomaly

1. "Entrez Gene: cytochrome P450". 2. Bailey LR, Roodi N, Dupont WD, Parl FF (1998). "Association of cytochrome P450 1B1 (CYP1B1) polymorphism with steroid receptor status in breast cancer.". Cancer Res. 58 (22): 5038-41. 3. Michels-Rautenstrauss KG, Mardin CY, Zenker M et al. (2002). "Primary congenital glaucoma: three case reports on novel mutations and combinations of mutations in the GLC3A (CYP1B1) gene.". J. Glaucoma 10 (4): 354-7.

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