Full Product Name
Anti-TRP1 Monoclonal Antibody
Product Gene Name
anti-TRP1 antibody
[Similar Products]
Product Synonym Gene Name
TYRP1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P17643
Specificity
No cross reactivity with other proteins.
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human TRP1 Oxidation of 5, 6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5, 6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-TRP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TRP1 antibody
Rabbit IgG Monoclonal antibody for P17643 detection. Tested positive for WB IHC IP in Human
Applications Tested/Suitable for anti-TRP1 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TRP1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50
Western Blot (WB) of anti-TRP1 antibody
Figure 1. Western blot analysis of TYRP1 using anti-TYRP1 antibody (M01959-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRP1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TYRP1.
NCBI/Uniprot data below describe general gene information for TRP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000541.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000550.2
[Other Products]
UniProt Primary Accession #
P17643
[Other Products]
UniProt Secondary Accession #
P78468; P78469; Q13721; Q15679[Other Products]
UniProt Related Accession #
P17643[Other Products]
Molecular Weight
60,724 Da
NCBI Official Full Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
NCBI Official Synonym Full Names
tyrosinase related protein 1
NCBI Official Symbol
TYRP1??[Similar Products]
NCBI Official Synonym Symbols
TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
??[Similar Products]
NCBI Protein Information
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Protein Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Synonym Protein Names
Catalase B; Glycoprotein 75
Protein Family
Telomere repeat-binding protein
UniProt Gene Name
TYRP1??[Similar Products]
UniProt Synonym Gene Names
CAS21 PublicationManual assertion based on opinion iniRef.2; TRP1 PublicationManual assertion based on opinion iniRef.1; TRP-1; TRP1??[Similar Products]
NCBI Summary for TRP1
This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]
UniProt Comments for TRP1
Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu2+ ions (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:22556244, PubMed:16704458). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin ().
Research Articles on TRP1
1. The rs387907171 SNP in TYRP1 exhibits strong allele frequency differences among islands in Northern Island Melanesia. Its absence from Bougainville, as well as the weak association with decreased hair color, indicates that additional alleles contribute to the blondism phenotype.
Precautions
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Disclaimer
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