Product Name
Tyrosinase-Related Protein-1 (TYRP-1), Monoclonal Antibody
Popular Item
Full Product Name
Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker)
Product Synonym Names
5; 6 dihydroxyindole 2 carboxylic acid oxidase; 6-dihydroxyindole-2-carboxylic acid oxidase; Associated with iris pigmentation; CAS2; Catalase B (CATB); DHICA oxidase; Glycoprotein75 (GP75); Melanoma antigen gp75; Tyrosinase-related protein 1 (TYRP1); TYRRP
Product Gene Name
anti-TYRP-1 antibody
[Similar Products]
Product Synonym Gene Name
TYRP1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P17643
Species Reactivity
Human, Mouse. Others not known.
Specificity
It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.?Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
Form/Format
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant full-length human TYRP1 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
9p23
Positive Control
SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-TYRP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TYRP-1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TYRP-1 antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
IHC-F: 5-10ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunohistochemistry (IHC) of anti-TYRP-1 antibody
Formalin-fixed, paraffin-embedded human Melanoma stained with TYRP1 Rabbit Recombinant MAb (TYRP1/1564R) using AEC Chromogen.
SDS-Page of anti-TYRP-1 antibody
SDS-PAGE Analysis of Purified TYRP1 Rabbit Recombinant Monoclonal Antibody (TYRP1/1564R) using AEC Chromogen.
NCBI/Uniprot data below describe general gene information for TYRP-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000541.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000550.2
[Other Products]
UniProt Primary Accession #
P17643
[Other Products]
UniProt Secondary Accession #
P78468; P78469; Q13721; Q15679[Other Products]
UniProt Related Accession #
P17643[Other Products]
NCBI Official Full Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
NCBI Official Synonym Full Names
tyrosinase related protein 1
NCBI Official Symbol
TYRP1??[Similar Products]
NCBI Official Synonym Symbols
TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
??[Similar Products]
NCBI Protein Information
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Protein Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Synonym Protein Names
Catalase B; Glycoprotein 75
UniProt Gene Name
TYRP1??[Similar Products]
UniProt Synonym Gene Names
; TRP-1; TRP1??[Similar Products]
NCBI Summary for TYRP-1
This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]
UniProt Comments for TYRP-1
Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu2+ ions (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:22556244, PubMed:16704458). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin ().
Product References and Citations for anti-TYRP-1 antibody
Orlow, S.J., et al. 1994. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex. J. Invest. Dermatol. 103: 196-201.
Research Articles on TYRP-1
1. The rs387907171 SNP in TYRP1 exhibits strong allele frequency differences among islands in Northern Island Melanesia. Its absence from Bougainville, as well as the weak association with decreased hair color, indicates that additional alleles contribute to the blondism phenotype.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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