Product Name
TYRP1, siRNA
Full Product Name
TYRP1 siRNA (Human)
Product Synonym Names
CAS2; TYRP; TYRRP; 5.6-dihydroxyindole-2-carboxylic acid oxidase; DHICA oxidase; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1
Product Gene Name
TYRP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P17643
Specificity
TYRP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TYRP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TYRP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TYRP1 sirna
siRNA to inhibit TYRP1 expression using RNA interference
Applications Tested/Suitable for TYRP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TYRP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000541.1
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NCBI GenBank Nucleotide #
NM_000550.2
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UniProt Primary Accession #
P17643
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UniProt Secondary Accession #
P78468; P78469; Q13721; Q15679[Other Products]
UniProt Related Accession #
P17643[Other Products]
Molecular Weight
60,724 Da
NCBI Official Full Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
NCBI Official Synonym Full Names
tyrosinase-related protein 1
NCBI Official Symbol
TYRP1??[Similar Products]
NCBI Official Synonym Symbols
TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
??[Similar Products]
NCBI Protein Information
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Protein Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Synonym Protein Names
Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1
Protein Family
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Gene Name
TYRP1??[Similar Products]
UniProt Synonym Gene Names
CAS2; TYRP; TYRRP; DHICA oxidase; TRP; TRP-1; TRP1??[Similar Products]
UniProt Entry Name
TYRP1_HUMAN
NCBI Summary for TYRP1
This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]
UniProt Comments for TYRP1
TYRP1: Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3); also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. Belongs to the tyrosinase family.
Protein type: Membrane protein, integral; Amino Acid Metabolism - tyrosine; EC 1.14.18.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 9p23
Cellular Component: melanosome membrane; melanosome; integral to membrane; endosome membrane
Molecular Function: protein binding; protein homodimerization activity; copper ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen; protein heterodimerization activity
Biological Process: melanin biosynthetic process; melanosome organization and biogenesis; melanocyte differentiation; acetoacetic acid metabolic process
Disease: Albinism, Oculocutaneous, Type Iii; Skin/hair/eye Pigmentation, Variation In, 11
Research Articles on TYRP1
1. These data suggest that UVB-stimulated Ucn1 contributes to TRP1 production via the transcription of both Nurr-1 and Nur77. Ucn1, produced in melanoma cells, acts on melanoma cells themselves in an autocrine manner.
Precautions
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Disclaimer
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