Full Product Name
DPYS siRNA (Rat)
Product Synonym Names
Dihydropyrimidinase; DHP; DHPase; Dihydropyrimidine amidohydrolase; Hydantoinase
Product Gene Name
DPYS sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q63150
Specificity
DPYS siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DPYS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DPYS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DPYS sirna
siRNA to inhibit DPYS expression using RNA interference
Applications Tested/Suitable for DPYS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DPYS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_113893.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031705.1
[Other Products]
UniProt Primary Accession #
Q63150
[Other Products]
UniProt Secondary Accession #
Q642F0[Other Products]
UniProt Related Accession #
Q63150[Other Products]
Molecular Weight
56,815 Da
NCBI Official Full Name
dihydropyrimidinase
NCBI Official Synonym Full Names
dihydropyrimidinase
NCBI Official Symbol
Dpys??[Similar Products]
NCBI Protein Information
dihydropyrimidinase
UniProt Protein Name
Dihydropyrimidinase
UniProt Synonym Protein Names
Dihydropyrimidine amidohydrolase; Hydantoinase
UniProt Gene Name
Dpys??[Similar Products]
UniProt Synonym Gene Names
DHP; DHPase??[Similar Products]
UniProt Entry Name
DPYS_RAT
NCBI Summary for DPYS
enzyme thatcatalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism [RGD, Feb 2006]
UniProt Comments for DPYS
DPYS: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6- dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD). DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Hydrolase; Other Amino Acids Metabolism - beta-alanine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.5.2.2
Cellular Component: cytosol
Molecular Function: thymine binding; amino acid binding; dihydropyrimidinase activity; zinc ion binding; uracil binding; phosphoprotein binding
Biological Process: uracil metabolic process; beta-alanine metabolic process; uracil catabolic process; thymine catabolic process; protein homooligomerization; pyrimidine base catabolic process; protein homotetramerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
