Tubulin beta-1 chain; TUBB1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse IgG1

86CT4.4.4

Mouse

This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.

Purified Mouse Monoclonal Antibody (Mab)

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-TUBB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB~~1:100~500
IHC~~1:10~50
IF~~1:10~50

Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35ug/lane). TBB1 (arrow) was detected using the purified Mab.(1ug/ml)

Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of TBB1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

50327

tubulin, beta 1 class VI

tubulin beta-1 chain

Tubulin beta-1 chain

TBB1_HUMAN

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]

TUBB1: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Interacts with RANBP10. Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: microtubule; cytoplasm

Molecular Function: GTPase activity; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; spindle assembly; transmembrane transport

Disease: Macrothrombocytopenia, Autosomal Dominant, Tubb1-related

Study of 18 functional hemostatic polymorphisms in mucocutaneous bleeding disorders. Antón AI, et al. Ann Hematol, 2010 Nov. PMID 20532885.
Natural product derivative Bis(4-fluorobenzyl)trisulfide inhibits tumor growth by modification of beta-tubulin at Cys 12 and suppression of microtubule dynamics. Xu W, et al. Mol Cancer Ther, 2009 Dec. PMID 19996274.
Genotype-phenotype relationship for six common polymorphisms in genes affecting platelet function from 286 healthy subjects and 160 patients with mucocutaneous bleeding of unknown cause. Martínez C, et al. Br J Haematol, 2009 Jun. PMID 19388931.
TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. Navarro-Nú?ez L, et al. Thromb Haemost, 2008 Dec. PMID 19132255.
Roles of beta-tubulin residues Ala428 and Thr429 in microtubule formation in vivo. Joe PA, et al. J Biol Chem, 2009 Feb 13. PMID 19074767.

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