Product Name
Tubulin Beta 1 (TUBb1), DIY ELISA Kit
Full Product Name
Tubulin Beta 1 (TUBb1) ELISA Kit DIY Materials
Product Gene Name
TUBb1 diy elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Tubulin Beta 1 (TUBb1). No significant cross-reactivity or interference between Tubulin Beta 1 (TUBb1) and analogues was observed.
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other
biological fluids.
Detection Range
1.56-100ng/mL
Reagent Contents
Capture
Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TUBb1 diy elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for TUBb1 diy elisa kit
Infection immunity
Application Notes for TUBb1 diy elisa kit
Main materials for "Do It Yourself ELISA Kit"
NCBI/Uniprot data below describe general gene information for TUBb1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074440.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001080971.2
[Other Products]
UniProt Related Accession #
A2AQ07[Other Products]
Molecular Weight
50,441 Da
NCBI Official Full Name
tubulin beta-1 chain
NCBI Official Synonym Full Names
tubulin, beta 1 class VI
NCBI Official Symbol
Tubb1??[Similar Products]
NCBI Official Synonym Symbols
M(beta)1; 2810484G07Rik
??[Similar Products]
NCBI Protein Information
tubulin beta-1 chain
UniProt Protein Name
Tubulin beta-1 chain
UniProt Gene Name
Tubb1??[Similar Products]
UniProt Comments for TUBb1
TUBB1: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Interacts with RANBP10. Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2|2 H4
Cellular Component: cytoplasm; microtubule
Molecular Function: structural constituent of cytoskeleton
Biological Process: microtubule-based process; spindle assembly
Research Articles on TUBb1
1. RanBP10 inhibits premature microtubule polymerization of beta1-tubulin and plays a pivotal role in thrombus stabilization.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
