Product Name
Tubulin Beta 1 (TUBb1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Tubulin Beta 1 (TUBb1)
Product Gene Name
anti-TUBb1 antibody
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Unconjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2007261)
Cy3 Conjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2075171)
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Unconjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2007261)
FITC Conjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2075172)
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HRP Conjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2075173)
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APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
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Unconjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2007261)
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Biotin Conjugated Antibody: Tubulin Beta 1 (TUBb1) (MBS2095808)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PYN AVLSIH QLIENTDACF CIDNEALYDI CFRTLRLTTP TYGDLNHLVS LTMSGITTSL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTAQGSQQ YRALSVAELT QQMFDARNIM AACDPRRGRY LTVACIFRGK MSTKEVDQQL LSIQTRNSNC FVEWIPNNVK VAVCDIPPRG LNMAATFLGN NTAIQELFTR VSEHFSAMFR RRAFVHWYTS EGMDISEFGE AESDIHDLVS EYQQFQDVRA GLEDSEE
3D Structure
ModBase 3D Structure for A2AQ07
Specificity
The antibody is a rabbit polyclonal antibody raised against TUBb1. It has been selected for its ability to recognize TUBb1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant TUBb1 (Pro182~Glu437) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075170
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-TUBb1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TUBb1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-TUBb1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB) of anti-TUBb1 antibody
Western Blot: Sample: Recombinant protein.

Immunohistochemistry (IHC) of anti-TUBb1 antibody
DABstainingonIHC-P.Samples:MouseTissue)

NCBI/Uniprot data below describe general gene information for TUBb1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074440.1
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NCBI GenBank Nucleotide #
NM_001080971.2
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UniProt Primary Accession #
A2AQ07
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UniProt Related Accession #
A2AQ07[Other Products]
Molecular Weight
50,441 Da
NCBI Official Full Name
tubulin beta-1 chain
NCBI Official Synonym Full Names
tubulin, beta 1 class VI
NCBI Official Symbol
Tubb1??[Similar Products]
NCBI Official Synonym Symbols
M(beta)1; 2810484G07Rik
??[Similar Products]
NCBI Protein Information
tubulin beta-1 chain
UniProt Protein Name
Tubulin beta-1 chain
UniProt Gene Name
Tubb1??[Similar Products]
UniProt Comments for TUBb1
TUBB1: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Interacts with RANBP10. Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2|2 H4
Cellular Component: cytoplasm; cytoskeleton; microtubule; microtubule cytoskeleton
Molecular Function: GTP binding; GTPase activity; nucleotide binding; structural constituent of cytoskeleton
Biological Process: microtubule-based process; spindle assembly
Research Articles on TUBb1
1. RanBP10 inhibits premature microtubule polymerization of beta1-tubulin and plays a pivotal role in thrombus stabilization.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.