For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_000010.10 (73724120..73773322). Location: 10q22.1

Store all reagents at 2-8 degree C

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Small volumes of CHST3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9300684 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbohydrate Sulfotransferase 3 (CHST3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHST3. The ELISA analytical biochemical technique of the MBS9300684 kit is based on CHST3 antibody-CHST3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

54,706 Da

carbohydrate (chondroitin 6) sulfotransferase 3

carbohydrate sulfotransferase 3; GST-0; C6ST-1; chondroitin 6-O-sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0

Carbohydrate sulfotransferase 3

C6ST-1; GST-0??[Similar Products]

CHST3_HUMAN

This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009]

CHST3: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N- acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen. Defects in CHST3 are a cause of spondyloepiphyseal dysplasia with congenital joint dislocations (SEDC-JD). A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; EC 2.8.2.17; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: chondroitin 6-sulfotransferase activity; sulfotransferase activity; proteoglycan sulfotransferase activity

Biological Process: chondroitin sulfate metabolic process; sulfur metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; T cell homeostasis

Disease: Spondyloepiphyseal Dysplasia With Congenital Joint Dislocations; Multiple Joint Dislocations, Short Stature, Craniofacial Dysmorphism, And Congenital Heart Defects

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