Monoclonal CYP7B1; Anti-CYP7B1; Cytochrome P450 family 7 subfamily B polypeptide 1; Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase; Cytochrome P450; Cytochrome P 450; Cytochrome P-450

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

7H10

Mouse

CYP7B1 antibody was purified by affinity chromatography.

Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.

500 ug-1 mg/ml (lot specific)

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Small volumes of anti-CYP7B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal CYP7B1 antibody

Proteases, Inhibitors, & Enzymes

Western Blot (WB)

WB: 1:1000

Western Blot analysis using CYP7B1 antibody
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CYP7B1 protein (Right) or empty vector (Left) detected with CYP7B1 antibody

56,496 Da

cytochrome P450, family 7, subfamily B, polypeptide 1

25-hydroxycholesterol 7-alpha-hydroxylase

CYP7B1 protein

Q05C57_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. [provided by RefSeq, Jul 2008]

CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.100; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 25-hydroxycholesterol 7alpha-hydroxylase activity; iron ion binding; heme binding; oxysterol 7-alpha-hydroxylase activity

Biological Process: bile acid biosynthetic process; cholesterol metabolic process; bile acid metabolic process; xenobiotic metabolic process; sterol metabolic process; transmembrane transport; positive regulation of epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway

Disease: Spastic Paraplegia 5a, Autosomal Recessive; Bile Acid Synthesis Defect, Congenital, 3

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.