Full Product Name
DAOA siRNA (Human)
Product Synonym Names
G72; D-amino acid oxidase activator; Protein G72
Product Gene Name
DAOA sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P59103
Specificity
DAOA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DAOA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DAOA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DAOA sirna
siRNA to inhibit DAOA expression using RNA interference
Applications Tested/Suitable for DAOA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DAOA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001155284.1
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NCBI GenBank Nucleotide #
NM_001161812.1
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UniProt Primary Accession #
P59103
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UniProt Secondary Accession #
Q0VAE6; Q5VX59; Q86Y17; Q8IWM4; A6NKG7[Other Products]
UniProt Related Accession #
P59103[Other Products]
Molecular Weight
14,719 Da
NCBI Official Full Name
D-amino acid oxidase activator isoform 2
NCBI Official Synonym Full Names
D-amino acid oxidase activator
NCBI Official Symbol
DAOA??[Similar Products]
NCBI Official Synonym Symbols
LG72; SG72
??[Similar Products]
NCBI Protein Information
D-amino acid oxidase activator
UniProt Protein Name
D-amino acid oxidase activator
UniProt Synonym Protein Names
Protein G72
Protein Family
D-amino acid oxidase activator
UniProt Gene Name
DAOA??[Similar Products]
UniProt Synonym Gene Names
G72??[Similar Products]
UniProt Entry Name
DAOA_HUMAN
NCBI Summary for DAOA
This gene encodes a protein that may function as an activator of D-amino acid oxidase, which degrades the gliotransmitter D-serine, a potent activator of N-methyl-D-aspartate (NMDA) type glutamate receptors. Studies also suggest that one encoded isoform may play a role in mitochondrial function and dendritic arborization. Polymorphisms in this gene have been implicated in susceptibility to schizophrenia and bipolar affective disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Mar 2011]
UniProt Comments for DAOA
DAOA: Seems to activate D-amino acid oxidase. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 13q33.2|13q34
Cellular Component: Golgi apparatus; mitochondrion; perinuclear region of cytoplasm
Molecular Function: enzyme binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity
Disease: Schizophrenia
Research Articles on DAOA
1. This study suggested that structural lipid alterations in oligodendrocyte glycosynapses are responsible for dysconnectivity in schizophrenia and that increased expression of G72 protein may play a role in the development of abnormal glycosynapses.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
